Endophthalmitis

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Endophthalmitis
Hypopyon with hazy media
Hypopyon with hazy media



Contents

Definition

Endophthalmitis is a purulent inflammation of the intraocular fluids (vitreous and aqueous) usually due to infection. 

  • Serious intraocular inflammatory disorder resulting from infection of the vitreous cavity
  • Progressive vitritis is the hallmark of any form of endophthalmitis
  • Histologically: massive infiltration of the vitreous cavity with inflammatory cells

Types of Endophthalmitis

Exogenous

  • Acute Postoperative
  • Chronic Postoperative
  • Traumatic
  • Filtering Bleb-Associated
  • After Intravitreal Injections
  • Corneal ulcer

Endogenous

  • Bacterial or fungal endogenous chorioretinitis +/- vitritis

Acute Postoperative Endophthalmitis

  • Refers to infectious endophthalmitis shortly after ocular surgery
  • Most present within 1-2 weeks, usually 3-5 days after the surgery.
  • Initial symptoms: rapidly progressive, including pain, red eye, ocular discharge, and blurring
  • Common signs: decreased visual acuity, lid swelling, conjunctival and corneal edema, anterior chamber cells + fibrin, hypopyon, vitreous inflammation, retinitis, and blunting of red reflex
  • Retinal Periphlebitis – earliest sign

Endophthalmitis Vitrectomy Study (EVS)[1]

EVS evaluated only the patients with acute endophthalmitis occuring within 6 weeks of cataract surgery or secondary intraocular lens implantation.

Symptoms

  • 94.3% reported blurred vision
  • 82.1% red eye
  • 74% pain- It is important that almost 25% patients did not have pain.
  • 34.5% swollen lid

Signs

  • 85% hypopyon- Fifteen percent patients may not have a hypopyon.
  • 79% hazy media
  • 26% LP vision only

When there is a doubt between post-operative inflammation and endophthalmitis with severe haze in the ocular media, or vitreous opacities clinically the possibility of an infection should be given priority given the potentially rapid and devastating consequences of delayed treatment.

Epidemiology

  • Acute postoperative endophthalmitis is the most common form of endophthalmitis
  • Following cataract surgery 0.08% - 0.68%
  • Rates increasing since clear corneal incisions
  • Highest risk after secondary IOL (0.2-0.367%), and lowest after pars plana vitrectomy (0.03-0.046%). Vitreous acts as a culture medium for micro-organisms, and hence after pars plana vitrectomy, the rates of endophthalmitis is least.

Evaluation

  • Suspect in any eye with inflammation greater than the usual postop course
  • Associations:
  • Wound leak or dehiscence- especially a leaking small incision cataract surgery (SICS) wound
  • Suture abscess
  • Vitreous incarceration in the wound
  • Eroding scleral sutures used to fixate IOLs

Presenting Visual Acuity

  • When measuring VA, the technique of differentiating LP vs. HM vision is most important
    • Pars plana vitrectomy recommended in patients with visual acuity of light perception (LP) or worse.
    • Hand motion (HM) or better, tap and inject intravitreal antibiotics . Source: https://www.eyeworld.org/
  • EVS gave the standard criteria to evaluate visual acuity in endophthalmitis : 'If no letters could be read on the (ETDRS) chart at 4 m, then at 1 m, vision was tested for the ability to count fingers. If the patient was unable to count fingers, vision was tested for the ability to recognize hand motions. For this, the patient's opposite eye was occluded, and a light source, such as a lamp used for near vision, was directed from behind the patient to the examiner's hand that either was stationary or was moved at one motion per second in a horizontal or vertical direction at a distance of 60 cm from the eye. The patient was asked to identify whether the examiner's hand was still, moving sideways, or moving up and down. The presentation was repeated five times, and hand-motion visual acuity was considered present if the patient was able to identify the examiner's action on at least four of the presentations. If the examiner was not convinced that hand motions could be detected, LP was tested at 0.9 m with an indirect ophthalmoscope set at maximum intensity.'

Differential Diagnosis of Acute Endophthalmitis

  1. Occult retention of lens cortex or nucleus
  2. Non-infectious hypopyon uveitis (Behcet’s or rifabutin)
  3. Infectious posterior uveitis (syphilitic, tuberculous, toxoplasmosis, toxocariasis in children)
  4. Non-infectious posterior uveitis (VKH, collagen vascular disorders, systemic vasculitides, idiopathic)
  5. Inflammatory reaction to intravitreal drug
  6. Blebitis
  7. Acute retinal necrosis
  8. Keratitis
  9. Toxic anterior segment syndrome (TASS)
    • Rapid onset (w/I 12-24 hours, limbus to limbus corneal edema)
    • Increased intraocular pressure
    • Absent vitreous inflammation
    • Iris damage
    • Repsonse to steroid
  10. Intraocular lymphoma (masquerade syndrome)

Testing

Ultrasound Evaluation

  • Should be performed if significant vitritis or media opacity prevents adequate view of the fundus
  • Findings c/w endophthalmitis:
    1. Dispersed vitreous opacities with vitritis
    2. Chorioretinal thickening
  • Assess for presence of: RD or choroidals, dislocated lens material, retained foreign bodies
  • Retinal or choroidal detachment are poor prognostic factors.

Microbiological Characteristics

  • Bacteria are the most common infecting agents
  • Causative organisms represent bacteria from patient’s own periocular flora, introduced during surgery. In cases of intravitreal medication associated endophthalmitis, causes include oral flora (treating physician or patient) or drug contamination. It is important therefore for these injections to not speak or to cover the treating physician with a mask and to ask the patient not to speak or use a drape.
  • In the EVS, 94.2% of culture-confirmed cases involved GP bacteria
  • 70% were GP, coag-negative staph (Staph epi)

Obtaining Specimens for Microbiologic Studies

  • In the EVS, vitreous samples yielded positive cultures more often than aqueous samples
  • Aqueous samples yielded positive culture a small minority (4.2%) of eyes
  • Given this, vitreous sampling is most often the first choice. Some surgeons obtain both vitreous and AC while others obtain AC sample if vitreous tap is dry. A minority obtain only an AC sample.
  • Eye should be prepped with 5% povidone-iodine. Lashes may be draped. An eyelid speculum may be used.
  • Subconjunctival or periocular lidocaine anesthesia usually required.

Vitreous Specimen

  • Needle aspiration
    • A 22 to 27G needle attached to a syringe is inserted into the vitreous cavity through the pars plana (pull plunger back first to break the vacuum before inserting into the eye).
    • Slowly aspirate. If no fluid vitreous can be obtained with a needle tap, an AC tap or vitreous biopsy must be performed instead to avoid aspirating formed vitrectomy
  • Vitrectomy
    • After conjunctival incision (if 20g), a vitrectomy probe attached to a TB syringe is inserted into the vitreous cavity through a sclerotomy placed 3-4mm posterior to limbus
    • Approx 0.1-0.3mL of vitreous is removed from the anterior vitreous cavity by using the automated cutting mechanism of the probe and slow, manual aspiration.

Anterior Chamber Specimen

  • A 27 to 30G needle is inserted near the limbus into the AC
  • 0.05 to 0.1cc of fluid is withdrawn.
  • Care should be taken to avoid the lens and iris. The AC should not be flattened. Do not aspirate the hypopyon as it is congealed will not go through the needle.

Specimen Handling

  • Culture inoculation by the surgeon or laboratory within minutes of obtaining specimens is ideal to maximize recovery of the organisms
  • Inject the specimen deeply into liquid media to ensure it the sample is within the media
  • Gram stain can help to determine the presence of infectious organisms and white cells acutely, cultures (aerobic, anaerobic, and fungal) are useful for specific identification of organisms and sensitivity
  • Antibiotic sensitivities (although in the EVS, Vanco and amikacin empiric tx covered 99.4% of all infecting organisms)
  • More recently, polymerase chain reaction (PCR) testing is being used more frequently to identify suspected pathogens in the sample. PCR testing requires the physician to specify the suspected organisms when requesting the test.

Treatment

Pars Plana Vitrectomy

  • Advantages: reduction of infecting organisms, toxins, inflammatory materials, and opacities. Allows collection of samples
  • Disadvantages: Need for sophisticated equipment and the need for an OR.
  • Vitrectomized eyes have more rapid clearance of intravitreal antibiotics
  • In inflamed, aphakic, vitrectomized rabits, intravitreal vanco and ceftaz half-lives are 7.5 and 5.1 hours, respectively, compared to 31.4 and 10.1 hours, respectively, in inflamed, phakic nonvitrectomized rabbits

PPV – obtaining sample

  • Standard 3 port setup
  • A vitreous sample of 0.2-0.5mL is first obtained without infusion using gentle manual aspiration into a syringe - this yields an undiluted vitreous specimen
  • Infusion is then initiated and core vitrectomy performed
  • The A/C is cleared for visualization as necessary
  • In most cases, IOL need not be removed

Aspiration of debris within the capsular bag if able to be performed safely is also helpful

  • In the EVS, attempts were made to clear 50% or more of the vitreous
  • Also in the EVS, no attempt to induce a PVD if none was previously present

Immediate PPV vs. Tap & Inject

  • In the EVS, patients who presented with LP only visual acuity had a significant, threefold improved chance of obtaining 20/40 vision after immediate vitrectomy (33%) compared to tap and inject (11%)
  • 56% of obtaining 20/100 or better after immediate PPV compared to 30% after tap
  • HM or better vision no significant difference between the treatment groups in final visual acuity (consider earlier PPV for diabetics)

EVS Limitations

  • Results may not apply to endophthalmitis after other ocular surgeries (EVS only studied post cataract and IOL surgery)
  • For example, post-intravitreal injection, bleb related endophthalmitis or chronic postoperative endophthalmitis were not studied in the EVS
  • Pts with NLP or significant opacification of A/C obscuring iris tissue were excluded from the study. Therefore severe infections or viruluent organisms were excluded
  • Unknown whether PPV is superior in these circumstances

Intravitreal Antibiotics

Current recommendations for empirical therapy (bacterial)

  • Vancomycin 1.0 mg/0.1 mL
  • Ceftazidime 2.25 mg/0.1 mL
  • Amikacin 400 ug/0.1 mL for Penicillin (PCN) allergic pts (although usually can still inject Ceftaz since there is low cross-reactivity between cephalosporins and PCN; keep in mind that most PCN "allergies" may not really be an allergic reaction; also low systemic absorption from ocular injection)
  • 4th generation fluoroquinolones – intravitreal moxifloxacin has been tried in some studies
  • Fungal: Amphotericin B 5-10 ug/0.1 mL or voriconazole 50-100 ug/0.1 mL

Complications of Intravitreal Antibiotics

  • Corneal opacification and retinal toxicity
  • Retinal toxicity with aminoglycosides, most notably gentamicin (even as low as 0.1mg)- macular infarction
  • Less common with amikacin and tobramycin
  • Amphotericin B – can also cause retinal toxicity
  • Hemorrhagic occlusive retinal vasculitis (HORV, rare but serious associated with vancomycin injection)[2][3]

Systemic Antibiotics

  • Usually too slow to enter the eye in adequate concentrations so not adequate for monotherapy. Some surgeons use adjunctive oral antibiotics (ie oral moxifloxacin) in addition to intravitreals
  • In the EVS, there was no difference in VA or media clarity with or without intravenous antibiotics (amikacin plus ceftazidime or amikacin plus ciprofloxacin) when given in addition to intravitreal antibiotics
  • However, did not have available newer 4th generation fluoroquinolones with better ocular penetration. Systemic ceftazidime, cefazoline, ciprofloxacin, gatifloxacin3 and moxifloxacin has been shown to penetrate the blood ocular barrier efficiently.

Corticosteroids

  • Goal is to modulate the host inflammatory response to the infection and minimize ocular damage from this response. Avoid when fungal infection is suspected.
  • Intravitreal corticosteroids (dexamethasone) is controversial
  • 2 retrospective nonrandomized studies showed 20/400 or better vision was obtained more in pts who received 400 ug adjuvant intravitreal dexamethasone
  • Randomized, prospective trial of 63 pts showed in no difference in VA at 12 weeks
  • Another retrospective, nonrandomized trial of 57 patients showed worse VA

Reinjection

A minority of patients will require further treatment, mainly for worsening ocular infection. It typically requires more than 24 hours to observe an improvement in the clinical appearance after initial treatment. In many cases, the treated eyes look somewhat worse 1 day after treatment before improving subsequently.

Considerations of reinjection

  • Should be considered if the infection fails to stabilize or improve more than 48 hours after the first injection
  • Based on consensus view, the EVS protocol recommended reinjection if the infection was worsening at 36-60 hours after initial injection
  • Often, 36 hours after treatment, culture results are available
  • Decision to reinject antibiotics should not be taken lightly, since repeat injection may increase risk of retinal toxicity
  • 100% retinal toxicity rates with 3 intravitreal doses of 1mg vanco combined with 400ug amikacin or 200 ug gent in rabbit eyes
  • Patients with no culture growth, equivocal growth, or coag-negative staph had a 5% rate of additional procedures, compared to 30% in pts with cultures that grew GN or other GP organisms

Outcomes and Risk Factors for Poor Outcomes

  • The most common cause of visual loss in the EVS was attributed to macular abnormalities, such as ERM, macular edema, pigmentary degeneration, and ischemia
  • Strongest predictor of poor visual outcome was light perception only vision
  • Other risk factors: older age, DM, corneal infiltrate or ring ulcer, compromised PC, low or high IOP, APD, rubeosis, and absent red reflex

Virulence

Coag-negative Staph were most likely to obtain 20/100 final VA or better (84%)Higher virulent organisms, such as Staph aureus, streptococcal species, and GN (Pseudomonus) organisms were associated with worse visual outcomes

Chronic Postoperative Endophthalmitis (> 6 weeks)

History

  • Usually manifest several weeks or months after surgery
  • Less common than acute variety
  • Organisms isolated are less virulent bacteria and fungi
  • 63% P acnes, 16% S epidermidis, 16% Candida parapsilosis

Clinical features

  • Pain or discomfort may not be present
  • Inflammation can be initially steroid responsive but recurrent after steroid taper
  • Fungal infections demonstrate paradoxical worsening with steroids
  • Frank hypopyon is often absent
  • Granulomatous uveitis with large precipitates on cornea or IOL
  • White intracapsular plaque with P acnes
  • Stringy white infiltrates and “fluff balls” or “pearls-on-a-string” near the capsular remnant are characteristic but not pathognomonic for fungal infection

Treatment for Chronic Cases

  • Obtain A/C and vitreous samples
  • Identification of infectious organism is key in management of these infections
  • Intravitreal injection
  • However, vancomycin is often inadequate for P acnes
  • Often need PPV and removal of capsule with IOL

Filtering Bleb-Associated Endophthalmitis

  • Occurs as a result of pathogenic organisms gaining entry to intraocular tissue through the conjunctival filtering bleb
  • Mean time between surgery and endophthalmitis is 19.1 months (range 3-9 years)
  • 0.2 – 9.6% of glaucoma filtering procedures
  • Increased incidence with use of antifibrotic agents
  • Thin, cystic, avascular conjunctiva
  • Blebitis – if no uveitis or vitritis

Post-Trabeculectomy Endophthalmitis Treatment

  • If only blebitis - conservative treatment with systemic and topical fortified antibiotic therapy
  • If the vitreous is clear, examine these patients frequently, treating for endophthalmitis if vitritis or acute hypopyon develops
  • Needle aspiration of the bleb is not advised
  • Studies suggest possible benefit from PPV

Endophthalmitis after Intravitreal Injection

  • Intravitreal injections have increased in volume worldwide, therefore prevalence of endophthalmitis has increased
  • Coagulase-negative staphylococci is the most common organism, similar to acute postop endophthalmitis
  • No infectious agent is identified in many cases
  • Triamcinolone acetonide crystals can migrate into A/C and mimic hypopyon
  • 1.4%/injection for IVK
  • 0.2%/injection for ranibizumab
  • Intravitreal triamcinolone may play a role in endophthalmitis potentiation

Non-infectious Endophthalmitis after Triamcinolone Injection or intravitreal anti-VEGF (aflibercept)[4]

  • Crystals in A/C or vitreous cavity can be difficult to distinguish from inflammatory reaction
  • Use gravity-induced shifting of layered material and absence of A/C flare or fibrin as a sign of a non-inflammatory cause
  • Triamcinolone crystals may shift from the inferior anterior chamber angle when the pt is placed on his or her side, a phenomenon that does not occur with sticky, fibrin-laden material in a true inflammatory hypopyon

Management options

  • Close observation, topical steroids alone, oral levofloxacin alone

Traumatic Endophthalmitis

Etiology

  • Approximately 25% of endophthalmitis cases
  • After open globe injury, chance of developing endophthalmitis is approx 7%
  • Injuries including IOFB have higher rates

Risk factors

  • Other risk factors: dirty wound, lens capsule rupture, age > 50, delayed presentation of more than 24 hours after injury
  • Bacillus and Staphylococcus are common in penetrating trauma with IOFB

Workup

  • Primary repair and removal of IOFB as soon as possible
  • Exclude the possibility of occult, retained IOFB
  • CT scan with thin 1mm cuts
  • Obtain cultures
  • Intravitreal Vanco and Ceftaz
  • Some advocate Gent + Clinda for synergistic effect against Bacillus and Staph

Treatment considerations

  • Application of EVS may not be appropriate, due to different organismsIn severe cases, vitrectomy should be strongly considered
  • Some authors advocate therapeutic vitrectomy in all cases of traumatic endophthalmitis
  • Others suggest performing vitrectomy only if pts do not respond after 48 hours or rapidly deteriorate in the first 24 hours following intravitreal antibiotics
  • Post-traumatic endophthalmitis has worst visual prognosis than other categories

Prophylaxis after Penetrating Trauma

  • Clinical evidence not established
  • Some authors advocate intravitreal antibiotic administration in all cases of penetrating trauma
  • Others recommend administration in the presence of risk factors (2 of 3: dirty wound, lens breach, or delay in closure over 24 hours)
  • Systemic antibiotics: Intravenous cefazolin (1g every 6 hours for 48-72 hours) followed by an oral agent such as gatifloxacin or moxifloxacin for 7 days

Endogenous Endophthalmitis

Etiology

  • Originates from sources within the body
  • 2-8% of endophthalmitis cases
  • Pts usually have underlying disease: History of recent intravenous infusion, DM, HIV, IV drug abuse, renal failure on dialysis, cardiac disease, malignancy, immunosuppressive therapy, or indwelling catheters
  • Liver abscess is the most common extraocular foci of infection, followed by pneumonia, endocarditis, soft tissue infection

Management of Endogenous Endophthalmitis

  • Cultures are essential and may be lifesaving
  • Vitreous sample should be obtained in all cases
  • ID of causatic organism is made by systemic culture of blood, urine or CSF in 75-80% of cases
  • In contrast to postoperative endophthalmitis, systemic antibiotics are central to the treatment of endogenous endophthalmitis
  • Focal chorioretinitis and associated mild vitritis can respond to systemic therapy alone

Conclusions

  • Early diagnosis and treatment with antimicrobial therapy are critical
  • Usually diagnosis must be made on clinical grounds
  • Treat with empirical therapy, usually Vanco + Ceftaz if bacterial
  • EVS showed that PPV was of benefit in pts with endophthalmitis following CE/IOL or secondary IOL placement who presented with LP only visual acuity
  • Although the role of vitrectomy in other forms of endophthalmitis is less clear, it remains an accepted adjunct to intravitreal antibiotics for tx of moderate to severe cases

References

  1. Endophthalmitis Vitrectomy Study Group. Results of the Endophthalmitis Vitrectomy Study. A randomized trial of immediate vitrectomy and of intravenous antibiotics for the treatment of postoperative bacterial endophthalmitis. Arch Ophthalmol 1995;113:1479–1496.
  2. Andreanos K, Petrou P, Kymionis G, Papaconstantinou D, Georgalas I. Early anti-VEGF treatment for hemorrhagic occlusive retinal vasculitis as a complication of cataract surgery. BMC Ophthalmol. 2017 Dec 6;17(1):238. doi:10.1186/s12886-017-0632-y. PubMed PMID:29212481; PubMed Central PMCID:PMC5719895.
  3. Witkin AJ, Chang DF, Jumper JM, Charles S, Eliott D, Hoffman RS, Mamalis N, Miller KM, Wykoff CC. Vancomycin-Associated Hemorrhagic Occlusive Retinal Vasculitis: Clinical Characteristics of 36 Eyes. Ophthalmology. 2017 May;124(5):583-595. doi: 10.1016/j.ophtha.2016.11.042. Epub 2017 Jan 19. PubMed PMID: 28110950.
  4. Grewal DS, Schwartz T, Fekrat S. Sequential Sterile Intraocular Inflammation Associated With Consecutive Intravitreal Injections of Aflibercept and Ranibizumab. Ophthalmic Surg Lasers Imaging Retina. 2017 May 1;48(5):428-431. doi: 10.3928/23258160-20170428-10. PubMed PMID: 28499055.
  1. Endophthalmitis, a review of current evaluation and management. Lemley et al. Retina 27:662-680, 2007
  2. Das T, Sharma S; Hyderabad Endophthalmitis Research Group.Current management strategies of acute post-operative endophthalmitis.Semin Ophthalmol. 2003;18(3):109-115