Optical Coherence Tomography Angiography

From EyeWiki
Original article contributed by: David Cordeiro Sousa
All contributors: David Cordeiro Sousa, Joao Breda, MD and Luis Abegão Pinto, MD, PhD
Assigned editor:
Review: Assigned status Up to Date by Joao Breda, MD on May 26, 2017.

Optical Coherence Tomography Angiography


Optical coherence tomography angiography (OCT-A) emerged as a non-invasive technique for imaging the microvasculature of the retina and choroid, and the first clinical studies using this innovative technology were published in 2014 .[1]

Briefly, OCT-A technology uses laser light reflectance of the surface of moving red blood cells to accurately depict vessels through different segmented areas of the eye, thus eliminating the need for intravascular dyes.[2] The OCT scan of a patient's retina consists in multiple individual A-scans, which compiled into a B-scan provides cross-sectional structural information. With OCT-A technology, the same tissue area is repeatedly imaged and differences analyzed between scans, thus allowing one to detect zones containing high flow rates (i.e. with marked changes between scans) and zones with slower, or no flow at all, which will be similar among scans.[3]

Light is emitted through either a spectral domain OCT (SD-OCT), with a wavelength of near 800nm; or a swept-source OCT (SS-OCT), which utilizes a longer wavelength, close to 1050nm. Longer wavelengths have a deeper tissue penetrance, but a slightly lower axial resolution. OCT-A employs two methods for motion detection: amplitude decorrelation or phase variance. The former detects differences in amplitude between two different OCT B-scans. Phase variance is related to the emitted light wave properties, and the variation of phase when it intercepts moving objects. To improve visualization and reduce background noise from normal small eye movements, two averaging methods - split spectrum amplitude decorrelation technique and volume averaging - were developed.[4][5] These OCT-A algorithms produce an image (3mm2 to 12mm2) that is segmented, by standard, into four zones: the superficial retinal plexus, the deep retinal plexus, the outer retina and the choriocapillaris. Applied to the optic disc it includes its full depth.[6][7]

Currently, there are currently 4 main commercially available OCT-A devices[8]:

  • ZEISS Angioplex™ OCT angiographic imaging on the CIRRUS™ HD-OCT platform, with a scanning rate up to 68,000 A-scans per second and an improved tracking software known as FastTrac™. A three-dimensional image is obtained depicting erythrocyte flow as well as the microvasculature of the superficial, deep, and avascular layers of the retina.
  • Optovue AngioVue® (Optovue, Inc., Freemont, CA), which uses split-spectrum amplitude-decorrelation angiography algorithm, which minimizes motion noise. This system also allows quantitative analysis, since it provides numerical data about flow area and flow density maps.
  • Topcon® uses a different algorithm, OCTA RatioAnalysis, which benefits from being paired with SD-OCT, and improves detection sensitivity of low blood flow and reduced motion artifacts without compromising axial resolution.
  • Heidelberg engineering® uses the active eye-tracking system (TruTrack™) that assesses simultaneously fundus and OCT images acquisition in order to achieve a better signal-to-noise ratio.
Figure 1 - Detailed microvasculature of the macula using OCT-A technology.
Figure 2 - Normal optic disc microvasculature evaluated using OCT-A.

Advantages over conventional angiography methods

Main advantages are the shorter acquisition time and being a non-invasive process, as fluorescein and indocyanine-green angiography require an injectable dye (which takes time to reach retinal vessels, and may be associated with systemic adverse effects and even anaphylatic reactions[2]).

One asset of this OCT-based approach is that it provides a quantitative analysis of the retinal vessels (in addition to the qualitative analysis done on standard Angiography). Moreover, and contrary to the "2-D" conventional angiograms, OCT-A technology provides "3-D" imaging information of the macula and visualizes peripapillary capillaries that supply the retinal nerve fiber layer.[6]

However, some limitations and artifacts are important to consider in the interpretation of OCT-A images (see section below).

Clinical Applications


As a fast, safe and noninvasive procedure to assess the chorioretinal microvasculature, OCT-A has been increasingly used in retinal diseases. The number of studies reporting new findings and utilities is exponentially growing. Therefore, only a brief description of current uses is available in this article and further information should be looked in the available ophthalmology journals.

OCT-A has been reported to be useful in the diagnosis and understanding of many retinal conditions, namely:

  • Diabetic retinopathy - identifying microaneurysms, neovascular complexes, and quantifying foveal avascular zone and nonperfused areas, showing good agreement with FA findings. [9][10][11]
  • Dry age-related macular degeneration - a general decrease in choriocapillaris flow has been reported, typically extending beyond the borders of areas of atrophy. Devices using SS-OCT are associated with better definition of choroidal vasculature changes.[12][13]
  • Wet age-related macular degeneration - qualitative and quantitative analysis of choroidal neovascular membranes (CNVM), being able to classify them, and to follow-up structural changes after intravitreal injections. It has also been raised the potential for detection of these neovascular complexes in non-exudative cases, which would be difficult to detect using SD-OCT or FA, and thus contribute to a more effective and closer follow-up.[14][15]
  • Central serous corioretinopathy - overlap between findings in FA of a pigmented epithelium detachment (PED) and CNVM may lead to situation of misdiagnosis. Especially in suspicious cases of flat and irregular PEDs, OCT-A may be helpful in diagnosis and management of CNVM.[16] Although some reports mention a decreased choriocapilaris blood flow[17][18], one should pay attention to signal strenght in en face images before interpreting this as hypoperfusion.
  • Vascular occlusions - evaluation of nonperfused areas and the integrity of superficial and deep plexus. The preservation of the deep vasculature has been associated with better visual outcomes.[19][20]
  • Macular telangiectasia - identification of the dilated, irregular telangiectatic vessels and, occasionally, the choroidal communication. Since OCT-A does not detect leakage, its combination with the OCT B-scan may help stage and manage MacTel without using FA.[21][22]
  • Choroidal neovascular membranes of miscellaneous causes (e.g. associated with high myopia), with good sensitivity and specificity for detection.[23]


OCT-A is also gaining increasing popularity for optic nerve disorders assessment, such as glaucoma.

It has been reported as a useful tool for evaluating optic disc perfusion in glaucomatous eyes, since attenuated peripapillary and macular vessel density was detectable in pre-perimetric glaucoma patients. Therefore, there is enthusiasm about the role of OCT-A in early detection of glaucomatous damage. Moreover, the quantitative data from these retinal vessels may prove useful in analysing metabolic activity from the inner layers of the retina and thus provide further advances in monitoring function and progression in this disease.[14][24]

It may also prove useful as a tool for ocular blood flow research and thus to help uncover non-IOP related mechanisms in this disease.

Figure 3 - OCT-A (left) and en face structural imaging (right) of a glaucoma patient, revealing inferior temporal loss of retinal fiber layer and the anatomical correspondence with decreased vessel density in the OCT-A image.  


Francesco Pichi and collaborators recently reviewed the uses and importance of OCT-A in uveitis.[8] For the sake of this article, only a brief summary will be reported.

The authors divided the findings among the standard layers obtained with OCT-A:

  • Superficial retinal capillary plexus - involved in inflammatory vasculitis, in which OCT-A is able to detect capillary dropout of superficial retinal vessels, capillary remodeling and a lower vessel density in uveitic eyes. The vascular reserve can be interpreted, which may be useful in management decisions. Also, in birdshot corioretinopathy, it has been suggested by recent OCT-A findings that the main ischemic insult responsible for secondary macular thinning may be in these inner retinal vessels.[25][26]
  • Deep retinal capillary plexus - although smaller changes were detected when compared to superficial plexus in inflammatory conditions, OCT-A is able to detect patterns associated with cystoid macular edema. However, one should always bear in mind the potential for artefacts in deep plexus visualisation (see below).[25][27]
  • Choriocapillaris - in inflammatory conditions associated with choroidal flow reduction or ischemia, OCT-A was able to detect flow void areas, that correlated with indocyanine-green angiography findings.[8]

The detection and evaluation of choroidal vascular membranes may be similarly achieved with OCT-A in uveitis, as in the other causes referred above.[28] Specifically in inflammatory conditions, OCT-A has the advantage of acquiring three-dimensional data, and potentially improving our understanding of the pathophysiology of these diseases as well as their follow-up and management. However, multimodal imaging is still the option of choice in the diagnosis and management of uveitis.[8]

Limitations & Artefacts

As a light source dependent device, some limitations arise for OCT-A technology. Media opacities can lead to signal attenuation and shadowing artifact.[3] Also, if one is looking for abnormalities in deeper vessels of the retina, it must be known that they may be obscured by superficial blood vessels.[5]

Projection artifact is inevitable and is related to light that traverses blood vessels and is reflected back by deeper layers (e.g. pigment retinal epithelium), which will appear in the final image with a similar vascular pattern as the overlying vessels.[29]

As stated above, OCT-A examination is extremely motion-sensitive and requires patient collaboration, which may be difficult to obtain for the visually impaired.[11]

Automated segmentation of structural abnormal retinas is also an unavoidable limitation of OCT-A imaging. In cases of PED, one should look carefully for segmentation errors and manually edit layers if deemed necessary for a correct interpretation.[30]

Moreover, and importantly, imaging the peripheral retina is mandatory in many cases, for both diagnosis and treatment. As such, the limited area of visualization (3mm2 to 12mm2) is a current limitation.[11] It should be noted that as the area increases, resolution drops markedly in order to keep scanning time acceptable.

Lastly, one should remember that OCT-A detects "motion", and thus very low amounts of blood flow near the device threshold may be undetected.[31]


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