File:Enhanced En Face OCT Image of Choroidal Vasculature Using Flattening and Slab Adjustments.jpg

To generate an en face OCT image of choroidal vessels using Topcon IMAGEnet software, start by acquiring a high-resolution 3D macular volume or wide-field scan, ensuring the scan is centered on the macula or posterior pole. Once the dataset is captured, access the en face imaging module and select the desired choroidal layer by adjusting the segmentation lines. This involves using the segmentation tool to define the lower boundary beneath Bruch's membrane, extending to the choroidal-scleral interface. If segmentation lines are misaligned, manually correct them to isolate the choroidal slab. Next, apply the flattening function to align the image to Bruch’s membrane (BM) or the retinal pigment epithelium (RPE), ensuring the en face image is evenly displayed and focused. Enhance the vessel visibility by adjusting the contrast and brightness or enabling the vessel enhancement filter, if available. Use the depth slider to scroll through en face views, setting a thinner slab thickness (e.g., 20–30 microns) for fine details or a thicker slab for broader vascular patterns. Apply averaging filters to reduce noise and improve clarity if needed. Once the desired visualization is achieved, save or export the image for further analysis. Additionally, enabling Enhanced Depth Imaging (EDI) and minimizing motion artifacts during acquisition further optimizes image quality for detecting choroidal anomalies like pachyvessels, vortex veins, or macroaneurysms. (Courtesy of J Khadamy)