Anterior Segment Optical Coherence Tomography Features of Keratitis

From EyeWiki


Optical coherence tomography (OCT) was initially developed for imaging and analyzing the macula and optic nerve, but it is also being utilized to image the ocular surface and anterior segment of the eye.[1] Anterior segment OCT (AS-OCT) produces high-resolution cross-sectional images in a quick and noninvasive manner creating an “optical biopsy” of various anterior segment structures.[1]

Figure 1A: Slit lamp photograph of the left eye demonstrates focal areas of limbal thickening inferiorly. The orange dashed line represents the cross section in 1B. Figure 1B: AS-OCT of the left eye demonstrates a “healing” appearance of PUK: a hyporeflective irregular epithelium (blue arrows) and disorganized hyperreflective stroma (red arrow).

Peripheral Ulcerative Keratitis

Peripheral ulcerative keratitis (PUK) comprises a group of inflammatory diseases that cause corneal epithelial ulceration and then stromal destruction leading to peripheral corneal thinning.[2] PUK is associated with many systemic inflammatory disorders, including rheumatoid arthritis, polyarteritis nodosa, inflammatory bowel disease, systemic lupus erythematosus, and anti-neutrophil cytoplasmic antibodies (ANCA) vasculitides,[3] but can also occur secondary to infectious processes or as an idiopathic phenomenon.

AS- OCT has been used to monitor disease activity and categorized PUK into three stages: 1) acute stage; 2) healing stage; and 3) healed stage.[2] On AS-OCT, the acute stage is characterized by an absent corneal epithelium and disorganized anterior stroma with heterogeneous stromal reflectivity and focal thinning.[2] The healing stage demonstrates a hyporeflective irregular epithelium and less heterogeneous hyperreflective stroma.[2] Finally, the healed stage reveals a hyporeflective irregular epithelium, a demarcation line between the hyporeflective epithelium and hyperreflective stroma, and irregular thickening.[2]

Figure 2A and 2B: AS-OCT of the right eye (left image) and AS-OCT of the left eye (right image) demonstrate healed PUK adjacent to the limbus in both eyes. Images show thinned hyporeflective epithelium (blue arrows) overlying focal areas of stromal hyperreflectivity (red arrows) with a sharp line of demarcation between the two regions (yellow arrows).

AS-OCT can also monitor the stabilization of corneal thinning with appropriate treatment.[4] Figure 1A depicts a slit lamp photograph and Figure 1B an AS-OCT image of a patient in the healing stage of PUK with the hyporeflective and irregular epithelium along with moderately heterogeneous stroma. The AS-OCT images in Figure 2 demonstrate characteristic findings in the healed stage of PUK: a thinned area of hyporeflective epithelium overlying heterogenous hyperreflective stroma with a sharp line of demarcation between these two areas.

Figure 3A: Slit lamp photo of the left eye at presentation with non-infectious interstitial keratitis showing focal areas of corneal haze. Dashed orange line is the plane of both AS-OCTs in 3B and 3C. Figure 3B: AS-OCT at presentation with focal areas of discrete mid-stromal hyperreflectivity with associated overlying and underlying stromal hyporeflectivity. Figure 3C: AS-OCT demonstrating more compact areas of mid-stromal hyperreflectivity (red arrows) after treatment with topical prednisolone and systemic methotrexate.

Non-Infectious Interstitial Keratitis

Interstitial keratitis is a non-ulcerative, non-suppurative, inflammatory reaction of the corneal stroma with or without associated neovascularization.[5] Also called stromal keratitis, this can be associated with localized or systemic infectious or non-infectious processes and is characterized by cellular and vascular infiltration of the stroma primarily with occasional secondary involvement of the corneal epithelium or endothelium.[5] The end result of interstitial keratitis is stromal disorganization which can lead to scar formation and reduced visual acuity from media opacity or induced astigmatism. Severe thinning can also be associated with a risk of spontaneous corneal perforation.

Figure 4A: Slit lamp photo of the right eye in a patient with inactive non-infectious interstitial keratitis. Dashed orange line is the plane of AS-OCT depicted in 4B. Figure 4B: AS-OCT image of the right eye demonstrating corneal thinning (yellow arrows) and deep stromal hyperreflectivity correlating with a stromal scar (red arrows)--a sequelae of interstitial keratitis.
Figure 7A: Slit lamp photo of the left eye demonstrating corneal haze in the area of prior keratitis. The dashed orange line represents the plane of AS-OCT in 7B. Figure 7B: AS-OCT demonstrating thickened hyporeflective epithelium (yellow arrows) and associated stromal disorganization (purple arrows) separated by a hyperreflective irregular boundary in this patient with healed microbial keratitis.

AS-OCT can be used to monitor treatment response as well as corneal thickness in areas of stromal keratitis.[6] Figure 3 demonstrates non-infectious interstitial keratitis of the same eye initially and then three months after treatment with topical prednisolone and systemic methotrexate. Figure 4 demonstrates the sequelae of interstitial keratitis–stromal thinning and a stromal scar.

Infectious Interstitial Keratitis

Figure 5A: Slit lamp photo of the left eye demonstrating a disrupted corneal epithelium with associated corneal infiltrate. The dashed orange line represents the plane of 5B. Figure 5B: AS-OCT image of the left eye showing thin, irregular hyperreflective epithelium and anterior stroma (red arrows) with an indistinct border between the two zones.
Figure 6A: Slit lamp photo of the left eye showing a new infiltrate adjacent to an area of prior corneal thinning in a patient with a neurotrophic cornea. The orange dotted line in 6A represents the plane of 6B. Figure 6B: AS-OCT image of the left eye demonstrating heaped hyperreflective epithelium (red arrows) adjacent to an area of irregular thin stroma from a prior episode of infectious keratitis (yellow arrows).
Figures 8A and 8C: Demonstrate slit lamp photos of the right and left eye, respectively. There is inferior corneal opacification (haze) and the dashed orange lines represent the cross-section of the corresponding AS-OCT images. Figure 8B: AS-OCT of the right eye shows a plaque involving the endothelium and deep stroma (red arrows) with a prominent hyperreflective line between the plaque and deep stroma (yellow arrows), which is more distinct than Descemet’s membrane. Figure 8D: OCT of the OS showing a similar albeit less thickened endothelial-stromal plaque with associated fine hyperreflective keratic precipitates (green arrows).
Figure 9A: Slit lamp photo of the right eye showing stromal thinning, scarring, and penetrating keratoplasty graft with intact sutures. The dashed orange line in 9A represents the cross-section of the AS-OCT image in 9B. Figure 9B: AS-OCT highlighting the corneal thinning without perforation (yellow double arrows) along with a sub-epithelial pseudocyst (red arrows).

Infectious keratitis can also cause vision loss via similar mechanisms as seen in non-infectious keratitis and can have viral, bacterial, fungal, or parasitic etiologies.[7] Risk factors for infectious keratitis include contact lens wear, trauma, and severe ocular surface disease.[6] Microbial keratitis is usually diagnosed based on a history of an inciting microtrauma to the epithelium and slit lamp examination identifying a corneal epithelial defect overlying a focal stromal infiltrate. AS-OCT can be used to monitor resolution or clinical decline in patients with infectious stromal keratitis. Figure 5 demonstrates active infectious keratitis with anterior stromal and epithelial hyperreflectivity and thinning. Figure 6 demonstrates bacterial keratitis adjacent to an area of prior stromal thinning. Figure 7 shows the sequelae of microbial keratitis, stromal thinning with a thickened epithelium.

Endothelial Plaques

AS-OCT can highlight endothelial pathology which may be a sequela of chronic uveitis. Figure 8 demonstrates an endothelial plaque with a clear boundary between the cornea and plaque. This suggests that plaque formation is from an intense inflammatory reaction resulting in a cluster of inflammatory material from the anterior chamber depositing on the corneal endothelium.[8]

Corneal Thinning/Perforation

Corneal perforations are ocular emergencies that can occur secondary to penetrating trauma in healthy corneas or mild insults in those with active or prior keratitis with associated stromal thinning. These patients may also have shallow/flat anterior chambers with aqueous egress (Seidel positivity). While detectable by slit lamp examination in most cases, AS-OCT can be used to assess areas which may be suspicious or difficult to view through areas of corneal haze with stromal thinning. Figure 9 demonstrates corneal thinning in a patient who had a prior penetrating keratoplasty for corneal perforation. AS-OCT shows a sub-epithelial cystoid change. Figure 10 depicts an area of ectasia found to be Seidel positive. The corresponding AS-OCT image highlights the posterior corneal ectasia with a focal tract and excavation leading to the ocular surface.

Figures 10A and 10B demonstrate slit lamp photos of the right eye. The dashed lines represent the cross-sections of the AS-OCT images (Figures 10C, 10D, 10E). The dashed line colors correspond to star colors. The red arrows in 10C show a focal path from the deep stroma anteriorly with only a thin area of overlying intact epithelium (blue arrows in 10D).


AS-OCT is becoming increasingly utilized for various ocular surface and anterior segment pathologies. We have highlighted various AS-OCT features of keratitis and some associated sequelae. More investigation can further characterize additional AS-OCT findings and better inform clinical decision-making.

  1. 1.0 1.1 Venkateswaran N, Galor A, Wang J, Karp CL. Optical coherence tomography for ocular surface and corneal diseases: a review. Eye Vis (Lond). 2018;5:13. doi:10.1186/s40662-018-0107-0
  2. 2.0 2.1 2.2 2.3 2.4 Bonnet C, Debillon L, Al-Hashimi S, et al. Anterior segment optical coherence tomography imaging in peripheral ulcerative keratitis, a corneal structural description. BMC Ophthalmol. May 25 2020;20(1):205. doi:10.1186/s12886-020-01466-1
  3. Fu L, Jones S. Peripheral Ulcerative Keratitis. [Updated 2021 Oct 12]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2022 Jan-. Available from:
  4. Garg A, De Rojas J, Mathews P, et al. Using Anterior Segment Optical Coherence Tomography to Monitor Disease Progression in Peripheral Ulcerative Keratitis. Case Rep Ophthalmol Med. 2018;2018:3705753. doi:10.1155/2018/3705753
  5. 5.0 5.1 Gauthier AS, Noureddine S, Delbosc B. Interstitial keratitis diagnosis and treatment. J Fr Ophtalmol. 2019;42(6):e229-e237. doi:10.1016/j.jfo.2019.04.001
  6. 6.0 6.1 Konstantopoulos A, Kuo J, Anderson D, Hossain P. Assessment of the use of anterior segment optical coherence tomography in microbial keratitis. Am J Ophthalmol. 2008;146(4):534-542. doi:10.1016/j.ajo.2008.05.030
  7. Durand ML, Barshak MB, Chodosh J. Infectious Keratitis in 2021. JAMA. 2021;326(13):1319-1320. doi:10.1001/jama.2021.0424
  8. Takezawa Y, Suzuki T, Shiraishi A. Observation of Retrocorneal Plaques in Patients With Infectious Keratitis Using Anterior Segment Optical Coherence Tomography. Cornea. 2017;36(10):1237-1242. doi:10.1097/ICO.0000000000001286
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